pE-340fura LED Illumination for Fura-2 Calcium Imaging
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Ships from:
United Kingdom
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On FindLight:
since 2020
Description
The pE-340 fura is a cutting-edge LED illuminator specifically designed for Fura-2 ratiometric calcium imaging. Built upon the successful pE-300 Series platform, this illuminator offers a compact and cost-effective solution for both specialized and everyday fluorescence microscopy. By providing optimal excitation wavelengths at 340 nm and 380 nm, the pE-340 fura ensures high-precision, stable, and high-throughput imaging capabilities with video-rate time resolution.
In practical applications, the pE-340 fura has demonstrated its effectiveness in capturing detailed images of cardiac myocytes, or heart cells, loaded with Fura-2. These images, obtained by researchers Martin Bootman and Katja Rietdorf from The Open University, highlight the illuminator's ability to support synaptically-driven Ca2+ events at varying frequencies, showcasing its versatility and precision in live-cell imaging.
One of the standout features of the pE-340 fura is its rapid response time. Unlike traditional illumination systems that rely on mechanical switching, the pE-340 fura can be controlled via BNC TTL connections, allowing for precise illumination control in as little as 20 microseconds. This advancement significantly enhances the speed and accuracy of Fura-2 imaging, making it a valuable tool for researchers.
Our expertise in LED technology has enabled us to optimize the internal optics of the pE-340 fura for efficient transmission of the 340 nm wavelength. The system is also configurable to accept a specific UV-compatible 3mm liquid light guide, ensuring high transmission efficiency. For those requiring additional customization, an optional Universal Collimator is available to further enhance the system's performance.
pE-340fura LED Illumination for Fura-2 Calcium Imaging
Specifications |
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| Emission Spectral Range: | 340 – 700 nm |
| Application: | Microscopy |
| Excitation Wavelength: | 340 nm & 380 nm |
| Precision: | better than 5 nM |
| Control Response Time: | 20 microseconds |
| Imaging Rate: | 0.5 Hz and 24.39 Hz |
Features
- Optimized for Fura-2 Imaging: The pE-340 fura is specifically designed for Fura-2 ratiometric calcium imaging, providing the ideal excitation wavelengths of 340 nm and 380 nm for high-precision imaging.
- High Throughput and Precision: Achieve stable, high-throughput imaging with video-rate time resolution, capturing synaptically-driven Ca2+ events at rates up to 24.39 Hz.
- Fast Illumination Control: The system offers precise illumination control with response times as fast as 20 microseconds via BNC TTL connections, surpassing traditional arc lamp systems.
- Compact and Affordable: Built on the successful pE-300 Series platform, the pE-340 fura offers a compact and cost-effective solution for both specialized and everyday fluorescence microscopy.
- Enhanced LED Technology: Utilizes advanced LED technology to ensure optimal transmission of 340 nm wavelengths, enhancing the quality and reliability of your imaging results.
- Configurable Options: Compatible with a UV compatible 3mm liquid light guide for high transmission efficiency, with an optional Universal Collimator available for further customization.
- Proven Performance: Backed by research and publications, the pE-340 fura delivers better than 5 nM precision in live cell imaging, ensuring reliable and reproducible results.
Applications
- Ratiometric Calcium Imaging: High-precision Fura-2 live-cell experiments
- Fluorescence Microscopy: Compatible with common fluorophores for everyday imaging
- Fast Kinetic Studies: Capture rapid calcium events with microsecond illumination switching
- Live-cell Imaging: Reduced dye toxicity improves cell viability and experimental reproducibility
- High-Throughput Experiments: Reliable, stable illumination for extended imaging sessions
Frequently Asked Questions
Yes, the pE-340fura LED Illuminator is compatible with most imaging software for integrated control with imaging set up.
The pE-340fura LED Illuminator has dedicated 340 nm and 380 nm outputs for Fura-2 ratiometric calcium imaging, broad white output (435 nm – 645 nm) for imaging standard fluorophores, microsecond switching (340/380) for capturing high-speed events, stable LED illumination minimizes noise, higher signal-to-noise gives cleaner images and data whilst requiring less Fura-2 dye, reducing toxicity and costs, UV optimized optics delivers maximum irradiance on standard microscope configurations, individual channel triggering via TTL in microseconds, and precise irradiance control in 1% steps (0-100%): no ND filters required.
The pE-340fura LED Illuminator provides the optimum excitation wavelengths for Fura-2-based calcium imaging allowing high-precision, stable, high-throughput imaging with video- rate time resolution.
The pE-340fura is a bespoke LED Illuminator used for Fura-2 ratiometric calcium imaging, which also supports everyday fluorescence microscopy in a compact and affordable package.
The pE-340fura LED Illuminator can be controlled via convenient BNC TTL connections for precise illumination control in as little as 20 microseconds.