Photometrics DVΛ - Dual-Channel Simultaneous-Imaging System
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Ships from:
United States
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Sold by:
Photometrics -
On FindLight:
since 2015
Description
The Photometrics DVΛ™ represents a cutting-edge advancement in the realm of simultaneous imaging systems for the life sciences. This dual-channel, simultaneous-imaging system is uniquely designed to enable the acquisition of two spatially identical but spectrally distinct images at the same time. This capability is particularly crucial for advanced research applications such as colocalization, ratiometric analysis, and polarization studies of a single emission wavelength channel. The DVΛ stands out from its predecessor, the DV2, by accommodating image sensors up to a 22 mm diagonal, making it compatible with a wide array of sCMOS sensors.
With its ability to separate emissions by wavelength, polarization, or amplitude, the DVΛ provides unparalleled flexibility in imaging applications. The system's exchangeable filter cube, which utilizes standard 25 mm diameter filters and polarizers, allows users to configure it for different filters and beam splitters, enhancing its versatility. Its design ensures easy mounting to most microscope side ports, and it includes a rectangular aperture to define a region of interest, making it a seamless addition to any laboratory setup.
The Photometrics DVΛ™ is engineered to deliver exceptional performance across a wavelength range of 450 to 900 nm, with transmission per image exceeding 88%. It operates efficiently within a temperature range of 10˚C to 37˚C, making it suitable for a variety of experimental conditions. The device's compact dimensions and lightweight design further enhance its practicality in research environments. By integrating seamlessly with leading microscopy packages, the DVΛ supports advanced image processing of DualView data, empowering researchers to achieve precise and reliable results in their imaging studies.
Photometrics DVΛ - Dual-Channel Simultaneous-Imaging System
Specifications |
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|---|---|
| Number Of Channels: | 2 |
| Sensor Type: | sCMOS |
| Wavelength Range: | 450 – 900 nm |
| Accommodates Image Sensors: | up to 22 mm diagonal |
| Transmission Per Image: | >88% |
| Operation Temperature: | 10˚C to 37˚C |
| Detector Attachment: | C-mount (male) |
| Front Attachment: | C-mount (female) |
| External Mounting Option: | ¼-20 tapped hole on back of unit |
| Dimensions: | 320 mm long, 110 mm wide, 79 mm tall |
| Weight: | 1.5 kg |
| Filter Diameter: | 25 mm |
| Recommended Filter Size: | 26x38x2 mm |
Features
- Accommodates Large Image Sensors: Supports image sensors up to 22 mm diagonal, including most sCMOS sensors.
- Simultaneous Dual-Channel Imaging: Enables acquisition of two full-field emission images at the same time.
- Versatile Emission Separation: Emission can be separated by wavelength, polarization, or amplitude.
- Customizable Filter Cubes: Uses standard 25 mm diameter filters and polarizers, configurable by the user for different filters and beam splitters.
- Easy Microscope Integration: Easily mounts to most microscope side ports for seamless integration.
- Region of Interest Definition: Incorporates a rectangular aperture to define a specific region of interest.
- Comprehensive Image Processing: DualView data processing is compatible with leading microscopy software packages.
- Wide Range of Applications: Ideal for FRET imaging, polarized FRET analysis, calcium imaging, fluorescence polarization/anisotropy imaging, and more.
- Optimal for Drug Discovery: Suitable for drug discovery applications using Cy3/Cy5.
- Advanced Imaging Techniques: Supports single-molecule fluorescence (SMF) imaging, pH imaging, multiwavelength TIRF imaging, and more.
- Robust Construction: Dimensions are 320 mm long, 110 mm wide, and 79 mm tall with a weight of 1.5 kg.
- Wide Wavelength Range: Operates effectively within a wavelength range of 450 to 900 nm.
- High Transmission Efficiency: Transmission per image is greater than 88% (excluding filter).
- Flexible Mounting Options: Includes C-mount (male) detector attachment, C-mount (female) front attachment, and an external mounting option with a ¼-20 tapped hole on the back of the unit.
- Temperature Range: Operates in a temperature range of 10˚C to 37˚C.
- Supports LightSpeed™ Mode: Compatible with Evolve™ 512 Delta with firmware newer than 12.2014.
Applications
- FRET Imaging: For analyzing molecular interactions via fluorescence resonance energy transfer.
- Polarization Studies: Enables fluorescence polarization and anisotropy imaging for structural analysis.
- Calcium and pH Imaging: Suitable for calcium imaging with fluo-3/Fura Red™ or dual-emission indo-1, and pH imaging with SNARF.
- Single-Molecule Fluorescence: Ideal for SMF imaging applications requiring high sensitivity.
- Multiwavelength TIRF Imaging: Supports total internal reflection fluorescence for advanced surface studies.
- Simultaneous Fluorescence/DIC Imaging: Capture fluorescence and differential interference contrast images concurrently.
- Drug Discovery Assays: Compatible with Cy3/Cy5 for high-throughput screening.
- Multichannel Confocal Microscopy: Enables multichannel imaging when used with spinning-disk confocal systems.
Frequently Asked Questions
The DVΛ can be used for fluorescence resonance energy transfer (FRET) imaging, polarized FRET analysis, calcium imaging, fluorescence polarization/anisotropy imaging, simultaneous fluorescence/DIC imaging, drug discovery, single-molecule fluorescence (SMF) imaging, pH imaging, multiwavelength total internal reflection fluorescence (TIRF) imaging, voltage sensing, fluorescence in situ hybridization (FISH) imaging, and multichannel confocal microscopy.
The DVΛ uses exchangeable filter cubes that can be configured by the user for different filters and beam splitters. Standard 25 mm diameter filters and polarizers can be used.
The Photometrics DVΛ is a dual-channel simultaneous-imaging system that allows a user to acquire two spatially identical but spectrally distinct images simultaneously.
The DVΛ can accommodate image sensors up to a 22 mm diagonal, while the DV2 cannot.
The DVΛ is 320 mm long, 110 mm wide, and 79 mm tall. It weighs 1.5 kg.