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- Photometrics DVΛ - Dual-Channel Simultaneous-Imaging System
Photometrics DVΛ - Dual-Channel Simultaneous-Imaging System
OVERVIEW
The DVΛ is an emission splitting system enabling a user to acquire two spatially identical but spectrally distinct images simultaneously. What distinguishes the DVΛ from the DV2 is that DVΛ can accommodate image sensors up to a 22 mm diagonal. Simultaneous, multichannel imaging is essential for colocalization, ratiometric analysis, or polarization studies of a single emission wavelength channel.
SPECIFICATION
- Number Of Channels: 2
- Sensor Type: sCMOS
- Wavelength Range: 450 - 900 nm
Applications
• Fluorescence resonance energy transfer (FRET) imaging
• Polarized FRET analysis
• Calcium imaging with fluo-3/Fura Red™ (Molecular Probes) or dual-emission indo-1 imaging
• Fluorescence polarization/anisotropy imaging
• Simultaneous fluorescence/DIC imaging
• Drug discovery with Cy3/Cy5
• Single-molecule fluorescence (SMF) imaging
• pH imaging with SNARF
• Multiwavelength total internal reflection fluorescence (TIRF) imaging
• Voltage sensing with di-4-ANEPPS
• Fluorescence in situ hybridization (FISH) imaging
• cAMP imaging with FICRhR
• Multichannel confocal microscopy when used in conjunction with a spinning-disk confocal
KEY FEATURES
• Accommodates image sensors up to 22 mm diagonal including most sCMOS sensors
• Simultaneous acquisition of two full-field emission images
• Emission can be separated by wavelength, polarization, or amplitude
• Exchangeable filter cube use standard 25 mm diameter filters and polarizers
• Filter cubes can be configured by the user for different filters and beam splitters
• Easily mounts to most microscope side ports
• Incorporates rectangular aperture to define a region of interest
• Image processing of DualView data is available in leading microscopy packages
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