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- Photometrics DC2 - Dual-Channel Simultaneous-Imaging System
Photometrics DC2 - Dual-Channel Simultaneous-Imaging System
OVERVIEW
The Photometrics® DC2™ enables two-channel simultaneous imaging based on two distinct wavelengths or polarizations. Each channel is projected onto an individual
CCD. Simultaneous multichannel imaging is essential to achieve quantitative emission ratiometric imaging.
SPECIFICATION
- Number Of Channels: 2
- Sensor Type: CCD
- Wavelength Range: 400 - 750 nm
Applications
• Fluorescence resonance energy transfer (FRET) imaging
• Calcium imaging with fluo-3/Fura Red™ (Molecular Probes) or dual-emission indo-1 imaging
• Fluorescence polarization/anisotropy imaging
• Simultaneous fluorescence/DIC imaging
• Drug discovery with Cy3/Cy5
• Single-molecule fluorescence (SMF) imaging
• pH imaging with SNARF
• Multiwavelength total internal reflection fluorescence (TIRF) imaging
• Voltage sensing with di-4-ANEPPS
• Fluorescence in situ hybridization (FISH) imaging
• cAMP imaging with FICRhR
• Multichannel confocal microscopy when used in conjunction with a spinning-disk confocal
KEY FEATURES
• Simultaneous acquisition of two full-field emission images
• Emission can be separated by wavelength, polarization, or amplitude
• Increases scanning speeds by a factor of two in high-content screening applications
• Easily mounts to most microscopes
• Improved adjustment control enables easier image alignment
• Exchangeable filter cube allows multiple applications to be run with minimal realignment
• Bypass mode allows user to send all of the emission light to a single camera
• Integrated, adjustable CCD mask minimizes ghosting
• Independent focus adjustment for each camera
• Works with many Photometrics® and QImaging® cameras*
*As some combinations of microscopes and objectives, particularly in
some newer Olympus and Zeiss stand/objective configurations, have
displayed vignetting, it is recommended that the DC2 be tested for
compatibility with your system. Please contact your local representative
to verify compatibility with specific cameras.
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