Frequently Asked Questions

The Photometrics DC2 can be used for fluorescence resonance energy transfer (FRET) imaging, calcium imaging with fluo-3/Fura Red or dual-emission indo-1 imaging, fluorescence polarization/anisotropy imaging, simultaneous fluorescence/DIC imaging, drug discovery with Cy3/Cy5, single-molecule fluorescence (SMF) imaging, pH imaging with SNARF, multiwavelength total internal reflection fluorescence (TIRF) imaging, voltage sensing with di-4-ANEPPS, fluorescence in situ hybridization (FISH) imaging, and cAMP imaging with FICRhR.

The Photometrics DC2 has a wavelength sensitivity of 400 to 750 nm, efficiency per image of 88 to 92%, operation temperature of 10 to 37˚C, detector attachment of C-mount (male), front attachment of C-mount (female), external mounting option of ¼-20 tapped hole on back of unit, dimensions of 6.5” at widest point x 8.5” height, weight of 4.8 lbs, and filters for emission/barrier, neutral density, and polarization with 1” (25.4-mm) max diameter and 0.39” (9.9-mm) max thickness.

The Photometrics DC2 features simultaneous acquisition of two full-field emission images, emission separation by wavelength, polarization, or amplitude, increased scanning speeds, easy mounting to most microscopes, improved adjustment control, exchangeable filter cube, bypass mode, integrated adjustable CCD mask, independent focus adjustment for each camera, and compatibility with many Photometrics and QImaging cameras.

The Photometrics DC2 works with many Photometrics and QImaging cameras, but it is recommended that the DC2 be tested for compatibility with your system, particularly in some newer Olympus and Zeiss stand/objective configurations, to avoid vignetting. Please contact your local representative to verify compatibility with specific cameras.

The Photometrics DC2 is a dual-channel simultaneous-imaging system that enables two-channel simultaneous imaging based on two distinct wavelengths or polarizations.

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