QuantEM 512SC EMCCD Camera for Life Sciences Imaging
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Ships from:
United States
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Sold by:
Photometrics -
On FindLight:
since 2015
Description
The Photometrics® QuantEM™ 512SC stands as a groundbreaking innovation in the realm of electron-multiplying CCD (EMCCD) cameras, specifically engineered for life sciences applications. As the first and only camera to offer on-chip signal amplification with true quantitative stability across 16 bits, the QuantEM™ 512SC is designed to meet the demanding needs of scientific research. With readout rates of 10, 5, and 1.25 MHz, this high-speed, high-performance camera enables researchers to conduct precise ratiometric analysis in time-course experiments, acquire reproducible data during long-term studies, and capture streaming data for multidimensional time-lapse investigations, all while maintaining single-molecule sensitivity.
At the heart of the QuantEM™ 512SC is a patent-pending PAR™ feedback system that ensures exceptional stabilization of EM gain. This innovative system, combined with an intelligent FPGA design, facilitates self-calibrating linearization of EM gain and prevents bias drift over time, ensuring a stable background and truly quantitative data. Furthermore, the advanced ACE™ technology provides superior timing resolution of the device’s pixel clocks, optimizing signal-to-noise sampling and minimizing spurious charge generation.
The camera's design includes a 512 x 512 imaging array with 16 x 16-μm pixels, offering a good field of view and sensitivity along with excellent resolution. The back-illuminated EMCCD ensures the highest available quantum efficiency, exceeding 90% peak QE. The QuantEM™ 512SC's dual amplifiers allow users to select the readout mode via software, optimizing for either high-speed/high-sensitivity performance or wide-dynamic-range performance. With a 16-bit digitization, this camera provides a wide dynamic range, capable of detecting both bright and dim signals within the same image.
Ease of integration is a key feature of the QuantEM™ 512SC, with a C-mount that easily attaches to microscopes, standard lenses, or optical equipment. The Turbo-1394™ interface ensures high-bandwidth, uninterrupted data transfer with no dropped frames. Compatible with Windows® XP/7 and supported by numerous third-party software packages, the QuantEM™ 512SC is a versatile and reliable tool for any life sciences laboratory.
QuantEM 512SC EMCCD Camera for Life Sciences Imaging
Specifications |
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|---|---|
| Sensor Type: | EMCCD |
| # Pixels (Width): | 512 |
| # Pixels (Height): | 512 |
| Pixel Size (Square): | 16 um |
| Peak Quantum Efficiency: | 92 % |
| Full Frame Rate: | 31.5 fps |
| Bit Depth: | 16 bit |
Features
- EM Gain: Provides very high sensitivity with a low-noise, impact-ionization process.
- Back-Illuminated EMCCD: Offers the highest available quantum efficiency (>90% peak QE).
- 512 x 512 Imaging Array: Features 16 x 16-μm pixels for a good field of view and sensitivity, along with good resolution.
- Intelligent FPGA Design: Ensures precise linearization of EM gain with self-calibrating capabilities to maintain truly quantitative data and prevent bias drift over time.
- PAR™ Feedback System: Delivers unsurpassed EM gain stability for outstanding signal fidelity across 16 bits.
- ACE™ Technology: Provides pixel-clock timing resolution 12x better than competing EMCCD cameras, offering the lowest noise floor and minimizing spurious charge and background events.
- 10-MHz Readout: Ideal for high-speed image visualization.
- 5- and 1.25-MHz Readout: Perfect for high-precision photometry.
- Dual Amplifiers: Allows selection of readout mode via software for optimized high-speed/high-sensitivity performance or wide-dynamic-range performance.
- 16-Bit Digitization: Offers a wide dynamic range, allowing detection of both bright and dim signals in the same image.
- Frame-Transfer EMCCD: Provides a 100% duty cycle to collect continuous data without the need for a mechanical shutter.
- C-Mount: Easily attaches to microscopes, standard lenses, or optical equipment.
- Turbo-1394™ Interface (IEEE-1394a): Ensures high-bandwidth, uninterrupted data transfer with no dropped frames.
- Windows® XP/7 Compatibility: Supported by numerous third-party software packages, with features like PVCam® circular buffers and device sequencing.
- Real-Time Focus: Allows precise integration with shutters, filter wheels, and more.
Applications
- Quantitative FRET: Ideal for precise Förster resonance energy transfer measurements.
- Multiprobe Experiments: Supports complex fluorescence assays requiring multiple probes.
- Ratiometric Ion Imaging: Enables accurate analysis of ion concentration changes.
- Confocal Microscopy: Delivers high-resolution, high-sensitivity imaging for advanced microscopy.
- Live-Cell Fluorescence Imaging: Suitable for time-lapse studies with single-molecule sensitivity.
Frequently Asked Questions
The QuantEM 512SC offers very high sensitivity, highest available quantum efficiency (>90% peak QE), good field of view and sensitivity, precise linearization of EM gain, unsurpassed EM gain stability for outstanding signal fidelity across 16 bits, and pixel-clock timing resolution 12x better than competing EMCCD cameras.
The QuantEM 512SC features a Turbo-1394™ interface (IEEE-1394a) for high-bandwidth, uninterrupted data transfer with no dropped frames. It is also supported by numerous third-party software packages.
The QuantEM 512SC is an electron-multiplying CCD (EMCCD) camera that offers on-chip signal amplification with true quantitative stability across 16 bits at readout rates of 10, 5, and 1.25 MHz.
The primary applications of the QuantEM 512SC include quantitative FRET, multiprobe experiments, ratiometric ion imaging, confocal microscopy, and live-cell fluorescence imaging.
The QuantEM 512SC offers readout rates of 10, 5, and 1.25 MHz.