Prime 95B Scientific CMOS Camera
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United States
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On FindLight:
since 2015
Description
The Prime 95B 22mm™ Scientific CMOS Camera represents a significant leap forward in scientific imaging technology, offering unparalleled sensitivity and precision. At the core of this innovation is its high Quantum Efficiency (QE) Backside Illumination (BSI) sensor, a groundbreaking first for Scientific CMOS cameras. This sensor is capable of converting up to an impressive 95% of incident photons into a measurable signal. Unlike traditional methods that rely on microlenses, which can lose their effectiveness at higher magnifications, the Prime 95B’s BSI technology ensures that light is efficiently directed into the pixel photodiode from behind, bypassing any structures that might reflect or absorb light. This results in over 300% more signal capture than other sCMOS cameras when used at 100X magnification, thanks to its large 11µm pixels.
The Prime 95B doesn’t just surpass other sCMOS cameras; it also outperforms EMCCD cameras by eliminating excess noise that often negates the benefits of a high QE sensor. Additionally, it avoids the complications associated with EM gain calibration, stability issues, high costs, and limited sensor lifetimes. With a true 16-bit dynamic range, the Prime 95B excels where EMCCD cameras fall short, detecting both weak and bright signals within the same image while maintaining photon-noise limited performance. This extreme sensitivity is not only crucial for detecting fainter signals, but it also provides the flexibility to increase frame rates or reduce excitation intensity, thereby minimizing cellular photo-damage.
Despite its advanced capabilities, the Prime 95B maintains the high frame rates, expansive field-of-view, and low read noise that have made sCMOS cameras a staple in live-cell imaging. Its ability to handle both dynamic and static scenes with precision makes it an ideal choice for various microscopy applications. Whether you're working with super-resolution microscopy, confocal microscopy, single molecule fluorescence, or light sheet microscopy, the Prime 95B ensures you capture the most detailed and accurate images possible.
Prime 95B Scientific CMOS Camera
Specifications |
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|---|---|
| Sensor Type: | sCMOS |
| # Pixels (Width): | 1200 |
| # Pixels (Height): | 1200 |
| Pixel Size (Square): | 11 um |
| Peak Quantum Efficiency: | 95 % |
| Full Frame Rate: | 41 fps |
| Bit Depth: | 12 bit |
| Read Noise: | 1.6 e- |
Features
- High Quantum Efficiency: Achieves 95% Peak QE, maximizing the ability to detect weak signals, enabling short exposure times for high frame rates, and minimizing phototoxicity across a wide range of wavelengths.
- Large 11µm Pixel Size: Maximizes light collection while maintaining proper spatial sampling, enhancing image quality.
- Extremely Low Read Noise: Enhances the ability to detect faint fluorescence, crucial for capturing subtle details in imaging.
- Fast Frame Rates: Allows capturing of highly dynamic events with high temporal resolution, offering up to 40fps at 16-bit and 80fps at 12-bit.
- Large Field of View: Maximizes the number of cells that can be tracked and monitored per frame, improving experimental efficiency.
- PrimeEnhanceTM: A real-time quantitative denoising algorithm that improves image clarity by reducing photon-shot (Poisson) noise, delivering a 3x to 5x increase in Peak Signal to Noise Ratio.
- PrimeLocateTM: Dynamically evaluates and acquires only the relevant data for localization-based super-resolution applications, optimizing data acquisition.
- Enhanced Dynamic Range: Measures both bright and dim signal levels within the same image, featuring a 50,000:1 Dynamic Range (94 dB).
- Multiple Expose Out Triggering: Controls up to four light sources for multi-wavelength acquisitions, enhancing experimental flexibility.
- SMART StreamingTM: Facilitates faster acquisition rates with variable exposures, ideal for multi-dimensional imaging.
Applications
- Super-Resolution Microscopy: Achieve high-resolution imaging beyond the diffraction limit.
- Confocal Microscopy: Enhance optical sectioning for detailed 3D reconstructions.
- Single Molecule Fluorescence: Detect and analyze individual fluorescent molecules with high sensitivity.
- Light Sheet Microscopy: Capture fast, volumetric images with minimized photodamage.
Frequently Asked Questions
The Prime 95B is a scientific CMOS camera with extreme sensitivity using high Quantum Efficiency (QE) Backside Illumination (BSI), a first for Scientific CMOS cameras.
The primary applications of the Prime 95B are Super-Resolution Microscopy, Confocal Microscopy, Single Molecule Fluorescence, and Light Sheet Microscopy.
The read noise of the Prime 95B is 1.6e- (median).
The Quantum Efficiency of the Prime 95B is 95%.
The pixel area of the Prime 95B is 11µm x 11µm.