The Prime 95B 22mm™ Scientific CMOS Camera represents a significant leap forward in scientific imaging technology, offering unparalleled sensitivity and precision. At the core of this innovation is its high Quantum Efficiency (QE) Backside Illumination (BSI) sensor, a groundbreaking first for Scientific CMOS cameras. This sensor is capable of converting up to an impressive 95% of incident photons into a measurable signal. Unlike traditional methods that rely on microlenses, which can lose their effectiveness at higher magnifications, the Prime 95B’s BSI technology ensures that light is efficiently directed into the pixel photodiode from behind, bypassing any structures that might reflect or absorb light. This results in over 300% more signal capture than other sCMOS cameras when used at 100X magnification, thanks to its large 11µm pixels.

The Prime 95B doesn’t just surpass other sCMOS cameras; it also outperforms EMCCD cameras by eliminating excess noise that often negates the benefits of a high QE sensor. Additionally, it avoids the complications associated with EM gain calibration, stability issues, high costs, and limited sensor lifetimes. With a true 16-bit dynamic range, the Prime 95B excels where EMCCD cameras fall short, detecting both weak and bright signals within the same image while maintaining photon-noise limited performance. This extreme sensitivity is not only crucial for detecting fainter signals, but it also provides the flexibility to increase frame rates or reduce excitation intensity, thereby minimizing cellular photo-damage.

Despite its advanced capabilities, the Prime 95B maintains the high frame rates, expansive field-of-view, and low read noise that have made sCMOS cameras a staple in live-cell imaging. Its ability to handle both dynamic and static scenes with precision makes it an ideal choice for various microscopy applications. Whether you're working with super-resolution microscopy, confocal microscopy, single molecule fluorescence, or light sheet microscopy, the Prime 95B ensures you capture the most detailed and accurate images possible.