Immersion Oil Type 37LDF
Description
Immersion Oil contributes to two characteristics of the image viewed through the microscope: finer resolution and brightness. These characteristics are most critical under high magnification; so it is only the higher power, short focus, objectives that are usually designed for oil immersion. Oil immersion objectives are generally available from 40 to 120x. These must not be confused with “high dry” objectives or water immersion objectives that are also made in this range. Just as an “oil” immersion objective must be used with oil to get a usable image, a “water” immersion objective must be used with water and a “dry” must be used dry. The use of oil on a high dry will destroy the image by negating corrections for spherical and chromatic aberration. For any given lens there is a fixed focal length. With the objective in focus there is a cone of light extending from a point on the specimen to the full diameter of the objective lens. The angle formed by this cone is the angular aperture (A.A.), shown diagrammatically in Figure 1. It may vary from 10° for low power dry (long focus), to 140° for high power oil (short focus). the greatest theoretical angular aperture is, of course, 180° with zero focal length. Below the specimen is a second, matching, cone of light, the base of the cone being the top surface of the condenser and the apex at a point on the specimen. Theoretical illumination, then, provides a straight line path for each ray from condenser to objective lens.
Immersion Oil Type 37LDF
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Frequently Asked Questions
Immersion Oil Type 37LDF contributes to finer resolution and brightness when viewing images through a microscope.
Oil immersion objectives are generally available from 40x to 120x.
No, oil immersion objectives must be used with oil to obtain a usable image. Water immersion objectives require water, and dry objectives must be used without any immersion medium.
Using oil on a high dry objective will destroy the image by negating corrections for spherical and chromatic aberration.
The angular aperture of high power oil immersion objectives can vary from 10° for low power dry objectives to 140° for high power oil objectives.