Immersion Oil Type B
Description
Immersion Oil contributes to two characteristics of the image viewed through the microscope: finer resolution and brightness. These characteristics are most critical under high magnification; so it is only the higher power, short focus, objectives that are usually designed for oil immersion. Oil immersion objectives are generally available from 40 to 120x. These must not be confused with “high dry” objectives or water immersion objectives that are also made in this range. Just as an “oil” immersion objective must be used with oil to get a usable image, a “water” immersion objective must be used with water and a “dry” must be used dry. The use of oil on a high dry will destroy the image by negating corrections for spherical and chromatic aberration. For any given lens there is a fixed focal length. With the objective in focus there is a cone of light extending from a point on the specimen to the full diameter of the objective lens. The angle formed by this cone is the angular aperture (A.A.), shown diagrammatically in Figure 1. It may vary from 10° for low power dry (long focus), to 140° for high power oil (short focus). the greatest theoretical angular aperture is, of course, 180° with zero focal length. Below the specimen is a second, matching, cone of light, the base of the cone being the top surface of the condenser and the apex at a point on the specimen. Theoretical illumination, then, provides a straight line path for each ray from condenser to objective lens.
Immersion Oil Type B
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