femtOgene Ultracompact Scanning Non-linear Microscope
Description
The femtOgene is an ultracompact scanning non-linear microscope with galvoscanners for beam scanning and focussing optics equipped with large-NA objectives (40x/1.3). Multiphoton effects in a sub-femtoliter focal volume induce a transient nanohole in the cellular membrane which allows optical nanoinjection of macromolecules including DNA, RNA, and proteins. The non-invasive gentle creation of a nanoopening without any collateral damage avoids cell death and enables fast self-repairing. Targeted transfection can be performed with high efficiency. femtOgene is based on a sub-20 femtosecond near infrared laser microscope with high-order dispersion compensation. The novel dispersion technology overcomes the problems of beam fluctuations observed in femtosecond laser systems based on prism technology. Nanoprocessing is performed in two exposure modes: (a) scanning of a region of interest (ROI) and (b) by single point illumination. Ablation, drilling and cutting can be performed with submicron accuracy. Targeted transfection and optical nanoinjection are based on millisecond exposure times and <10 mW mean power at 85 MHz repetition frequency.
femtOgene Ultracompact Scanning Non-linear Microscope
Specifications |
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|---|---|
| Scan Range (X): | 350 um |
| Scan Range (Y): | 350 um |
| Scan Range (Z): | 200 um |
| Spatial Resolution (lateral): | N/A um |
| Spatial Resolution (vertical): | N/A um |
Applications
Nanojoule sub-20 fs laser pulses at 85 MHz repetition rate can be used to perform targeted transfection and optical nanoinjection of macromolecules as well as optical knock-out of intracellular organelles. Of major interest is the transfection of stem cells. Stem cells may revolutionize current therapy such as gene therapy and tissue engineering. Genetically modified stem cells can be used for the production of immune system mediator proteins. femtOgene has been employed for the efficient targeted transfection of human salivary gland and pancreas stem cells.
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Frequently Asked Questions
Nanojoule sub-20 fs laser pulses at 85 MHz repetition rate can be used to perform targeted transfection and optical nanoinjection of macromolecules as well as optical knock-out of intracellular organelles.
The femtOgene can be used for gene therapy, stem cell manipulation, optical nanoinjection of macromolecules, optical knock-out of cellular organelles, intracellular chromosome dissection, and high resolution imaging.
The dimensions of the femtOgene system are: stand: 490 × 280 × 480 mm3, scan module: 280 × 190 × 90 mm3, control unit: 450 × 300 × 130 mm3, femtosecond laser: 507 × 280 × 81 mm3 (laser head) 483 × 280 × 88 mm3 (user interface) 175 × 104 × 102 mm3 (dispersion management modul).
The femtOgene is an ultracompact scanning non-linear microscope with galvoscanners for beam scanning and focussing optics equipped with large-NA objectives (40x/1.3).
The power requirements for the femtOgene are 230 VAC (50 Hz) or 115 VAC (60 Hz).