MCS MOM Computer System and Software
Description
The MOM Computer System (MCS) includes the software package MScan. This program has been designed to seamlessly control two-photon imaging using conventional or resonant scanners while incorporating photostimulation and electrophysiology. While designed exclusively for use with the MOM microscope, it is also compatible with other two-photon platforms. The MCS is designed to take on complex experiments in deep-tissue intravital imaging. Its intuitive user interface is easy to use. The MCS package and the MOM together form a formidable tool to understand the most complex issues in neuroscience, immunology or oncology. Importantly, you will find in MCS the same standard of technical excellence that is the hallmark of all Sutter Instrument products.
The MScan 2.0 software has been developed to simplify the many tasks inherent in complicated imaging experiments. MScan 2.0 is extensively multithreaded to take advantage of multicore processors. This ensures reliability and user interface responsiveness. Furthermore, MScan is multiuser based to facilitate sharing of a MOM microscope with MCS among experimenters. Experimenters can then send their data to other workstations for analysis. The MCS analysis program MView is available for free download on the Sutter Instrument website.
MCS includes a windows 7 workstation, National Instruments data acquisition boards, a USB camera and a USB controlled MPC-200. The National Instruments boards included are a PCI-6110 board for imaging, a PCIe-6353 board for control of imaging and photostimulation laser power and a PCIe-6321 board for electrophysiology. The package is a turnkey system as all data acquisition boards and software come installed within the workstation.
An important feature in MScan is the ability to do bidirectional frame scanning with sub-pixel line offset adjustment. Conventional two-photon frame scanning has involved unidirectional scanning. In these scenarios, data is only recorded when sweeping in one direction across the sample. To increase the rate of data acquisition, it is then necessary to steer the laser beam back to the origin of the scan as quickly as possible to begin the subsequent line. As galvanometric scanners are most taxed and most likely to be damaged during these high-frequency movements, bidirectional scanning both increases the speed at which frames can be recorded and decreases the likelihood of damage to expensive galvanometers.
MCS MOM Computer System and Software
Features
- Large-scale, high-resolution, deep-tissue mapping
- Multispectral, high-speed, functional optical imaging
- Resonant scanning capable with imaging rates to 30 frames per second
- Photostimulation while imaging through the same optical pathway ("photostimaging")
- Two-photon microscopy and concurrent electrophysiology with computer-controlled placement of electrodes by Sutter micromanipulators
Applications
- Two-photon imaging
- Deep tissue imaging
- Neurophysiology
- Immunology
- Embryology
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Frequently Asked Questions
Yes, the MCS MOM Computer System is designed to be compatible with other two-photon platforms.
The MCS MOM Computer System is suitable for applications such as two-photon imaging, deep tissue imaging, neurophysiology, immunology, and embryology.
The bidirectional frame scanning feature in MScan allows for faster data acquisition and decreases the likelihood of damage to expensive galvanometers.
The MCS MOM Computer System is a turnkey system that includes the software package MScan, designed to control two-photon imaging, photostimulation, and electrophysiology.
The MScan 2.0 software is a program developed to simplify the many tasks inherent in complicated imaging experiments. It is extensively multithreaded to take advantage of multicore processors.